A sensitive urinary lipoarabinomannan test for tuberculosis

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Abstract

We have previously developed a diagnostic test for tuberculosis based on detection of mycobacterial lipoarabinomannan (LAM) in urine. The method depended on a laborious concentration step. We have now developed an easy to perform test based on a magnetic immunoassay platform, utilizing high avidity monoclonal antibodies for the detection of LAM in urine. With this method the analytical sensitivity of the assay was increased 50-100-fold compared to conventional ELISA. In a pilot study of HIV-negative patients with microbiologically verified TB (n=17) and healthy controls (n=22) the sensitivity of the test was 82% and the specificity 100%. This is in stark positive contrast to a range of studies using available commercial tests with polyclonal anti-LAM Abs where the sensitivity of the tests in HIV-negative TB patients was very low.

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Hamasur, B., Bruchfeld, J., VanHelden, P., Källenius, G., & Svenson, S. (2015). A sensitive urinary lipoarabinomannan test for tuberculosis. PLoS ONE, 10(4). https://doi.org/10.1371/journal.pone.0123457

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