Short-term PTH administration increases dentine apposition and microhardness in mice

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Abstract

Objective: The purpose of this study is to investigate the effects of intermittent parathyroid hormone (PTH) administration on the apposition rate and structural features of dentine from mouse incisors. Methods: Young male A/J Unib mice were treated daily for 6 and 10 days with 40 μg/kg of hPTH 1-34 or a vehicle. Dentine apposition rates measured by fluorescent labels (tetracycline and calcein) and alkaline phosphatase (ALP) plasma levels were evaluated after 6 days of treatment. Knoop microhardness testing and element content measurements in at.% of calcium (Ca), phosphorus (P), oxygen (O), and magnesium (Mg) in the peritubular and intertubular dentine were performed by Energy Dispersive X-ray (EDX) microanalysis via Scanning Electron Microscopy (SEM) after 10 days of treatment. Results: Histometric analysis revealed an increase of 5% in the apposition rate of dentine and 25% in the ALP plasma levels in the PTH treated group. In addition, knoop microhardness testing revealed that the animals treated with PTH had a greater microhardness (11%). EDX microanalysis showed that PTH treatment led to increases in P (23%) and Ca (53%) at.% content, as well as the Ca/P ratio (24%) in peritubular dentine. The chemical composition of intertubular dentine did not vary between the groups. Conclusions: These findings indicate that intermittent administration of hPTH (1-34) increases apposition and mineralization of the dentine during young mice incisor formation. © 2012 Elsevier Ltd.

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Guimarães, G. N., Cardoso, G. B. C., Naves, L. Z., Correr-Sobrinho, L., Line, S. R. P., & Marques, M. R. (2012). Short-term PTH administration increases dentine apposition and microhardness in mice. Archives of Oral Biology, 57(10), 1313–1319. https://doi.org/10.1016/j.archoralbio.2012.03.007

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