Significance of four methionine sulfoxide reductases in Staphylococcus aureus

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Abstract

Staphylococcus aureus possesses three MsrA enzymes (MsrA1, MsrA2, MsrA3) that reduce the S-epimer of methionine sulfoxide (MetO) and an MsrB enzyme that reduces R-MetO. The four msr genes are expressed from three different promoters. The msrA1/msrB genes are coexpressed. To determine the expression pattern of msr genes, three independent reporter strains were constructed where msr promoter was cloned in front of a promoterless lacZ and the resulting construct was integrated in the chromosome. Using these strains, it was determined that the msrA1/B expression is significantly higher in S. aureus compared to msrA2 or msrA3 . Expression of msrA1/B was highest during stationary phase growth, but the expression of msrA2 and msrA3 was highest during the early to midexponential growth phase. Expression of msrA1/B was induced by oxacillin and the expression of msrA3 was upregulated by salt. Expression of msrA2 remained unchanged under all tested conditions.

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APA

Singh, V. K., Vaish, M., Johansson, T. R., Baum, K. R., Ring, R. P., Singh, S., … Moskovitz, J. (2015). Significance of four methionine sulfoxide reductases in Staphylococcus aureus. PLoS ONE, 10(2). https://doi.org/10.1371/journal.pone.0117594

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