We report on a silicon wafer-based device that can be used for recording macroscopic ion channel protein activities across a diverse group of cell-types. Gigaohm seals were achieved for CHO-K1 and RIN m5F cells, and both cell-attached and whole-cell mode configurations were also demonstrated. Two distinct intrinsic potassium ion channels were recorded in whole-cell mode for HIT-T15 and RAW 264.7 cells. Polydimethylsiloxane (PDMS) microfluidics were also coupled with the micromachined silicon chips in order to demonstrate that a single cell could be selectively directed to a micropore, and membrane protein currents could subsequently be recorded. These silicon chip-based devices have significant advantages over traditional micropipette approaches, and may serve as combinatorial tools for investigating membrane biophysics, pharmaceutical screening, and other bio-sensing tasks. © 2004 Elsevier B.V. All rights reserved.
Pantoja, R., Nagarah, J. M., Starace, D. M., Melosh, N. A., Blunck, R., Bezanilla, F., & Heath, J. R. (2004). Silicon chip-based patch-clamp electrodes integrated with PDMS microfluidics. In Biosensors and Bioelectronics (Vol. 20, pp. 509–517). https://doi.org/10.1016/j.bios.2004.02.020