Silicon chip-based patch-clamp electrodes integrated with PDMS microfluidics

147Citations
Citations of this article
106Readers
Mendeley users who have this article in their library.

Abstract

We report on a silicon wafer-based device that can be used for recording macroscopic ion channel protein activities across a diverse group of cell-types. Gigaohm seals were achieved for CHO-K1 and RIN m5F cells, and both cell-attached and whole-cell mode configurations were also demonstrated. Two distinct intrinsic potassium ion channels were recorded in whole-cell mode for HIT-T15 and RAW 264.7 cells. Polydimethylsiloxane (PDMS) microfluidics were also coupled with the micromachined silicon chips in order to demonstrate that a single cell could be selectively directed to a micropore, and membrane protein currents could subsequently be recorded. These silicon chip-based devices have significant advantages over traditional micropipette approaches, and may serve as combinatorial tools for investigating membrane biophysics, pharmaceutical screening, and other bio-sensing tasks. © 2004 Elsevier B.V. All rights reserved.

Cite

CITATION STYLE

APA

Pantoja, R., Nagarah, J. M., Starace, D. M., Melosh, N. A., Blunck, R., Bezanilla, F., & Heath, J. R. (2004). Silicon chip-based patch-clamp electrodes integrated with PDMS microfluidics. In Biosensors and Bioelectronics (Vol. 20, pp. 509–517). https://doi.org/10.1016/j.bios.2004.02.020

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free