A simple non-invasive method for measuring gross brain size in small live fish with semi-transparent heads

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Abstract

This paper describes a non-invasive method for estimating gross brain size in small fish with semi-transparent heads, using system camera equipment. Macrophotographs were taken fromabove on backlit free-swimming fish undergoing light anaesthesia. From the photographs, the width of the optic tectum was measured. This measure (TeO-measure) correlates well with the width of the optic tectum as measured from out-dissected brains in both brown trout fry and zebrafish (Pearson r > 0.90). The TeO-measure also correlates well with overall brain wet weight in brown trout fry (r = 0.90), but less well for zebrafish (r = 0.79). A non-invasive measure makes it possible to quickly assess brain size froma large number of individuals, as well as repeatedly measuring brain size of live individuals allowing calculation of brain growth.

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Näslund, J. (2014). A simple non-invasive method for measuring gross brain size in small live fish with semi-transparent heads. PeerJ, 2014(1). https://doi.org/10.7717/peerj.586

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