An HPLC method for the simultaneous quantitative determination of betamethasone and clotrimazole in cream formulation has been developed. The method utilizes a reversed-phase C18 (250 X 4.0 mm) stationary phase, with a mixture of methanol-acetate buffer-acetonitrile (33:27:40, v/v) as a mobile phase, and spectrophotometric UV detection at 254 nm. The method has been validated for cream formulations containing betamethasone and clotrimazole with linear range of 0.025 to 0.075 mg/ml for betamethasone with a correlation coefficient of 0.9996, and linear range of 0.25 to 0.75 mg/ml for clotrimazole with a correlation coefficient of 1.000. The results demonstrated that this method is accurate, precise, specific, linear, reliable, sensitive, and fast.
Manassra, A., Khamis, M., el-Dakiky, M., Abdel-Qader, Z., & Al-Rimawi, F. (2010). Simultaneous HPLC Analysis of Betamethasone and Clotrimazole in Cream Formulations. Pharmaceutica Analytica Acta, 01(02). https://doi.org/10.4172/2153-2435.1000113