A Single Mammalian Mitochondrial Translation Initiation Factor Functionally Replaces Two Bacterial Factors

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Abstract

The mechanism of translation in eubacteria and organelles is thought to be similar. In eubacteria, the three initiation factors IF1, IF2, and IF3 are vital. Although the homologs of IF2 and IF3 are found in mammalian mitochondria, an IF1 homolog has never been detected. Here, we show that bovine mitochondrial IF2 (IF2mt) complements E. coli containing a deletion of the IF2 gene (E. coli ΔinfB). We find that IF1 is no longer essential in an IF2mt-supported E. coli ΔinfB strain. Furthermore, biochemical and molecular modeling data show that a conserved insertion of 37 amino acids in the IF2mt substitutes for the function of IF1. Deletion of this insertion from IF2mt supports E. coli for the essential function of IF2. However, in this background, IF1 remains essential. These observations provide strong evidence that a single factor (IF2mt) in mammalian mitochondria performs the functions of two eubacterial factors, IF1 and IF2. © 2008 Elsevier Inc. All rights reserved.

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Gaur, R., Grasso, D., Datta, P. P., Krishna, P. D. V., Das, G., Spencer, A., … Varshney, U. (2008). A Single Mammalian Mitochondrial Translation Initiation Factor Functionally Replaces Two Bacterial Factors. Molecular Cell, 29(2), 180–190. https://doi.org/10.1016/j.molcel.2007.11.021

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