Single-cell electric lysis on an electroosmotic-driven microfluidic chip with arrays of microwells

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Abstract

Accurate analysis at the single-cell level has become a highly attractive tool for investigating cellular content. An electroosmotic-driven microfluidic chip with arrays of 30-μm-diameter microwells was developed for single-cell electric lysis in the present study. The cellular occupancy in the microwells when the applied voltage was 5 V (82.4%) was slightly higher than that at an applied voltage of 10 V (81.8%). When the applied voltage was increased to 15 V, the cellular occupancy in the microwells dropped to 64.3%. More than 50% of the occupied microwells contain individual cells. The results of electric lysis experiments at the single-cell level indicate that the cells were gradually lysed as the DC voltage of 30 V was applied; the cell was fully lysed after 25 s. Single-cell electric lysis was demonstrated in the proposed microfluidic chip, which is suitable for high-throughput cell lysis. © 2012 by the authors; licensee MDPI, Basel, Switzerland.

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APA

Jen, C. P., Amstislavskaya, T. G., Liu, Y. H., Hsiao, J. H., & Chen, Y. H. (2012). Single-cell electric lysis on an electroosmotic-driven microfluidic chip with arrays of microwells. Sensors (Switzerland), 12(6), 6967–6977. https://doi.org/10.3390/s120606967

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