Small Intestine Inflammation in Roquin-Mutant and Roquin-Deficient Mice

Citations of this article
Mendeley users who have this article in their library.


Roquin, an E3 ubiquitin ligase that localizes to cytosolic RNA granules, is involved in regulating mRNA stability and translation. Mice that have a M199R mutation in the Roquin protein (referred to as sanroque or Roquin san/san mice) develop autoimmune pathologies, although the extent to which these occur in the intestinal mucosa has not been determined. Here, we demonstrate that Roquin san/san mice reproducibly develop intestinal inflammation in the small intestine but not the colon. Similarly, mice generated in our laboratory in which the Roquin gene was disrupted by insertion of a gene trap cassette (Roquin gt/gt mice) had small intestinal inflammation that mimicked that of Roquin san/san mice. MLN cells in Roquin san/san mice consisted of activated proliferating T cells, and had increased numbers of CD44 hi CD62L lo KLRG1 + short-lived effector cells. Proportionally more small intestinal intraepithelial lymphocytes in Roquin san/san mice expressed the ICOS T cell activation marker. Of particular interest, small intestinal lamina propria lymphocytes in Roquin san/san mice consisted of a high proportion of Gr-1 + T cells that included IL-17A + cells and CD8 + IFN-γ + cells. Extensive cytokine dysregulation resulting in both over-expression and under-expression of chemotactic cytokines occurred in the ileum of Roquin san/san mice, the region most prone to the development of inflammation. These findings demonstrate that chronic inflammation ensues in the intestine following Roquin alteration either as a consequence of protein mutation or gene disruption, and they have implications for understanding how small intestinal inflammation is perpetuated in Crohn's disease (CD). Due to the paucity of animal models of CD-like pathophysiology in the small intestine, and because the primary gene/protein defects of the Roquin animal systems used here are well-defined, it will be possible to further elucidate the underlying genetic and molecular mechanisms that drive the disease process. © 2013 Schaefer et al.




Schaefer, J. S., Montufar-Solis, D., Nakra, N., Vigneswaran, N., & Klein, J. R. (2013). Small Intestine Inflammation in Roquin-Mutant and Roquin-Deficient Mice. PLoS ONE, 8(2).

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free