Soluble IL-1 receptor type I binds to human dermal fibroblasts and induces calcium flux

11Citations
Citations of this article
5Readers
Mendeley users who have this article in their library.

Abstract

Soluble cytokine receptors appear to modify ligand concentrations by stabilizing ligands or by specifically inhibiting interactions of ligands with their membrane-bound receptors. Here we describe a new function of the soluble interleukin-1 receptor type I (IL-1sR I). This receptor induced a transient rise of intracellular free calcium concentration in human dermal fibroblasts in a dose-dependent fashion. Mobilization of calcium by IL-1sR I was abolished in the presence of an equimolar concentration of IL-1 receptor antagonist (IL-1ra). Neutralizing antibodies against IL-1β also abolished calcium mobilization stimulated with IL-1sR I indicating that IL-1β is involved. IL-1sR I bound with high affinity (K(d) 1-2 nM) to the fibroblasts. In addition, IL-1sR I enhanced expression of IL-6 and IL-8 mRNA. The observation that IL-1sR I can act as a ligand and agonist for membrane IL-1 extends the concept of the ligand-receptor functions of both IL-1 and IL-1sR I and adds a new dimension to the cytokine network. Copyright (C) 1998 Federation of European Biochemical Societies.

Cite

CITATION STYLE

APA

Spörri, B., Wiesmann, U. N., Ochsenbein, R. M., Loetscher, M., Waelti, E. R., Lottaz, D., & Bickel, M. (1998). Soluble IL-1 receptor type I binds to human dermal fibroblasts and induces calcium flux. FEBS Letters, 434(3), 283–288. https://doi.org/10.1016/S0014-5793(98)00995-8

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free