Solvent isotope and viscosity effects on the steady-state kinetics of the flavoprotein nitroalkane oxidase

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Abstract

The flavoprotein nitroalkane oxidase catalyzes the oxidative denitrification of a broad range of primary and secondary nitroalkanes to yield the respective aldehydes or ketones, hydrogen peroxide and nitrite. With nitroethane as substrate the D2O(kcat/KM) value is 0.6 and the D2Okcat value is 2.4. The kcat proton inventory is consistent with a single exchangeable proton in flight, while the kcat/KM is consistent with either a single proton in flight in the transition state or a medium effect. Increasing the solvent viscosity did not affect the kcat or kcat/K M value significantly, establishing that nitroethane binding is at equilibrium and that product release does not limit kcat. © 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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Gadda, G., & Fitzpatrick, P. F. (2013). Solvent isotope and viscosity effects on the steady-state kinetics of the flavoprotein nitroalkane oxidase. FEBS Letters, 587(17), 2785–2789. https://doi.org/10.1016/j.febslet.2013.04.021

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