Structure of the biotinyl domain of acetyl-coenzyme A carboxylase determined by MAD phasing

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Background: Acetyl-coenzyme A carboxylase catalyzes the first committed step of fatty acid biosynthesis. Universally, this reaction involves three functional components all related to a carboxybiotinyl intermediate. A biotinyl domain shuttles its covalently attached biotin prosthetic group between the active sites of a biotin carboxylase and a carboxyl transferase. In Escherichia coli, the three components reside in separate subunits; a biotinyl domain is the functional portion of one of these, biotin carboxyl carrier protein (BCCP). Results We have expressed natural and selenomethionyl (Se-met) BCCP from E. coli as biotinylated recombinant proteins, proteolyzed them with subtilisin Carlsberg to produce the biotinyl domains BCCPsc and Se-met BCCPsc, determined the crystal structure of Se-met BCCPsc using a modified version of the multiwavelength anomalous diffraction (MAD) phasing protocol, and refined the structure for the natural BCCPsc at 1.8 å resolution. The structure may be described as a capped β sandwich with quasi-dyad symmetry. Each half contains a characteristic hammerhead motif. The biotinylated lysine is located at a hairpin β turn which connects the two symmetric halves of the molecule, and its biotinyl group interacts with a non-symmetric protrusion from the core. Conclusion This first crystal structure of a biotinyl domain helps to unravel the central role of such domains in reactions catalyzed by biotin-dependent carboxylases. The hammerhead structure observed twice in BCCPscmay be regarded as the basic structural motif of biotinyl and lipoyl domains of a superfamily of enzymes. The new MAD phasing techniques developed in the course of determining this structure enhance the power of the MAD method. © 1995 Elsevier Science Ltd. All rights reserved.




Athappilly, F. K., & Hendrickson, W. A. (1995). Structure of the biotinyl domain of acetyl-coenzyme A carboxylase determined by MAD phasing. Structure, 3(12), 1407–1419.

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