The Structure of the Karrikin-Insensitive Protein (KAI2) in Arabidopsis thaliana

35Citations
Citations of this article
67Readers
Mendeley users who have this article in their library.

Abstract

KARRIKIN INSENSITIVE 2 (KAI2) is an α/β hydrolase involved in seed germination and seedling development. It is essential for plant responses to karrikins, a class of butenolide compounds derived from burnt plant material that are structurally similar to strigolactone plant hormones. The mechanistic basis for the function of KAI2 in plant development remains unclear. We have determined the crystal structure of Arabidopsis thaliana KAI2 in space groups P212121(a = 63.57 Å, b = 66.26 Å, c = 78.25 Å) and P21(a = 50.20 Å, b = 56.04 Å, c = 52.43 Å, β = 116.12°) to 1.55 and 2.11 Å respectively. The catalytic residues are positioned within a large hydrophobic pocket similar to that of DAD2, a protein required for strigolactone response in Petunia hybrida. KAI2 possesses a second solvent-accessible pocket, adjacent to the active site cavity, which offers the possibility of allosteric regulation. The structure of KAI2 is consistent with its designation as a serine hydrolase, as well as previous data implicating the protein in karrikin and strigolactone signalling. © 2013 Bythell-Douglas et al.

Cite

CITATION STYLE

APA

Bythell-Douglas, R., Waters, M. T., Scaffidi, A., Flematti, G. R., Smith, S. M., & Bond, C. S. (2013). The Structure of the Karrikin-Insensitive Protein (KAI2) in Arabidopsis thaliana. PLoS ONE, 8(1). https://doi.org/10.1371/journal.pone.0054758

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free