The isolation of Clostridium botulinum from honey samples is described. Botulism is characterized as an intoxication provoked by ingestion of contaminated foods with this toxin. Infant botulism happens by the ingestion of spores of C. botulinum together with food that in special conditions of the intestinal tract, such as those present in babies of less than 1 year old, will allow the germination and colonization of the intestine with production and absorption of botulinic toxin. The samples were subjected to dilution and to a thermal shock and cultivated in modified CMM (Difco). Cultures were subjected to Gram smears and toxicity tests in mice. The toxic cultures were purified in RFCA (Oxoid) plates and incubated in anaerobic jars. Positive samples were typed using the mouse assay neutralization test. From the 85 honey samples analyzed, six were positive for C. botulinum (7.06%), and identified as producers of type A, B, and D toxins.
Schocken-Iturrino, R. P., Carneiro, M. C., Kato, E., Sorbara, J. O. B., Rossi, O. D., & Gerbasi, L. E. R. (1999). Study of the presence of the spores of Clostridium botulinum in honey in Brazil. FEMS Immunology and Medical Microbiology, 24(3), 379–382. https://doi.org/10.1016/S0928-8244(99)00057-7