Subspecific kDNA probes for major clones of Trypanosoma cruzi

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Abstract

Among the T. cruzi clones, a limited no. are repeatedly sampled over large geog. areas and long periods of time. These widespread clones have been named major clones: their medical and epidemiol. characteristics deserve to be investigated with utmost priority. Results concerning DNA probes specific for 2 of the major clones are presented. The polymerase chain reaction (PCR) was carried out in order to generate selectively important amts. of high variable regions (HVRm) from the kDNA minicircles of T. cruzi. Two hundred and fifty bp major HVRm fragments from the total DNA of 26 genetically highly diversified T. cruzi stocks, isolated from various places and hosts were successfully amplified. The sensitivity of PCR was estd. by titrn.; the minimal amt. of total DNA used as template was 10-4 fg. Moreover, lysed cells were tested by PCR, a pos. amplification was obtained from as few as 1-5 cells (epimastigote or trypomastigote forms). [on SciFinder(R)]

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Veas, F., Cuny, G., Brenière, S. F., & Tibayrenc, M. (1990). Subspecific kDNA probes for major clones of Trypanosoma cruzi. Acta Tropica, 48(1), 79–82. https://doi.org/10.1016/0001-706X(90)90067-A

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