Sulfur-regulated control of the met-2+ gene of Neurospora crassa encoding cystathionine β-lyase

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Abstract

Cystathionine β-lyase performs an essential role in the transsulfuration pathway by its primary reaction of forming homocysteine from cystathionine. Understanding how the Neurospora crassa met-2 + gene, which encodes cystathionine β-lyase, is regulated is important in determining the basis of the cellular control of transsulfuration. The aim of this study was to determine the nature of a potential regulatory connection of met-2 + to the Neurospora sulfur regulatory network. The cystathionine β-lyase (met-2 + ) gene was cloned by the identification of a cosmid genomic clone capable of transforming a met- 2 mutant to methionine prototrophy and subsequently characterized. The gene contains a single intron and encodes a protein of 457 amino acids with conserved residues predicted to be important for catalysis and pyridoxal-5′-phosphate co-factor binding. The expression of met-2 + in wild-type N. crassa increased 3.1-fold under sulfur-limiting growth conditions as compared to the transcript levels seen under high sulfur growth conditions (i.e., repressing conditions). In a Δcys-3 strain, met-2 + transcript levels were substantially reduced under either low- or high-sulfur growth conditions. In addition, the presence of CYS3 activator binding sites on the met-2 + promoter was demonstrated by gel mobility shift assays. In this report, we demonstrate the sulfur-regulated expression of the met-2+ gene and confirm its connection to the N. crassa sulfur regulatory circuit by the reduced expression observed in a Δcys-3 mutant and the in vitro detection of CYS3 binding sites in the met-2 + promoter. The data further adds to our understanding of the regulatory dynamics of transsulfuration.

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Reveal, B. S., & Paietta, J. V. (2013). Sulfur-regulated control of the met-2+ gene of Neurospora crassa encoding cystathionine β-lyase. BMC Research Notes, 6(1). https://doi.org/10.1186/1756-0500-6-259

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