The misdiagnosis of Plasmodium knowlesi by microscopy has prompted a re-evaluation of the geographic distribution, prevalence and pathogenesis of this species using molecular diagnostic tools. In this report, a specific probe for P. knowlesi, that can be used in a previously described TaqMan real-time PCR assay for detection of Plasmodium spp., and Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae and Plasmodium ovale, was designed and validated against clinical samples.
Divis, P. C., Shokoples, S. E., Singh, B., & Yanow, S. K. (2010). A TaqMan real-time PCR assay for the detection and quantitation of Plasmodium knowlesi. Malaria Journal, 9(1). https://doi.org/10.1186/1475-2875-9-344