A TaqMan real-time PCR assay for the detection and quantitation of Plasmodium knowlesi

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Abstract

The misdiagnosis of Plasmodium knowlesi by microscopy has prompted a re-evaluation of the geographic distribution, prevalence and pathogenesis of this species using molecular diagnostic tools. In this report, a specific probe for P. knowlesi, that can be used in a previously described TaqMan real-time PCR assay for detection of Plasmodium spp., and Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae and Plasmodium ovale, was designed and validated against clinical samples.

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Divis, P. C., Shokoples, S. E., Singh, B., & Yanow, S. K. (2010). A TaqMan real-time PCR assay for the detection and quantitation of Plasmodium knowlesi. Malaria Journal, 9(1). https://doi.org/10.1186/1475-2875-9-344

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