Targeted gene transfection from microbubbles into vascular smooth muscle cells using focused, ultrasound-mediated delivery

67Citations
Citations of this article
43Readers
Mendeley users who have this article in their library.
Get full text

Abstract

We investigated a method for gene delivery to vascular smooth muscle cells using ultrasound triggered delivery of plasmid DNA from electrostatically coupled cationic microbubbles. Microbubbles carrying reporter plasmid DNA were acoustically ruptured in the vicinity of smooth muscle cells in vitro under a range of acoustic pressures (0 to 950 kPa) and pulse durations (0 to 100 cycles). No effect on gene transfection or viability was observed from application of microbubbles, DNA or ultrasound alone. Microbubbles in combination with ultrasound (500-kPa, 1-MHz, 50-cycle bursts at a pulse repetition frequency [PRF] of 100 Hz) significantly reduced viability both with DNA (53 ± 27%) and without (19 ± 8%). Maximal gene transfection (∼1% of cells) occurred using 50-cycle, 1-MHz pulses at 300 kPa, which resulted in 40% viability of cells. We demonstrated that we can locally deliver DNA to vascular smooth muscle cells in vitro using microbubble carriers and focused ultrasound. © 2010 World Federation for Ultrasound in Medicine & Biology.

Cite

CITATION STYLE

APA

Phillips, L. C., Klibanov, A. L., Wamhoff, B. R., & Hossack, J. A. (2010). Targeted gene transfection from microbubbles into vascular smooth muscle cells using focused, ultrasound-mediated delivery. Ultrasound in Medicine and Biology, 36(9), 1470–1480. https://doi.org/10.1016/j.ultrasmedbio.2010.06.010

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free