T-cell apoptosis in human glioblastoma multiforme: Implications for immunotherapy

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We used immunohistochemistry and flow cytometry to assess apoptosis in human glioblastoma multiforme (GBM). Our immunohistochemical study revealed apoptosis of glioma cells expressing glial fibrillary acidic protein and of CD3+ T cells infiltrating GBM. To quantify and phenotype the apoptotic T cells, we performed flow cytometry on lymphocytes separated from GBM. The cells were stained with annexin-V-FLUOS/propidium iodide to identify apoptosis. We found that high proportions of both the CD4+ and CD8+ T cells were apoptotic. In particular, we found that T cells expressing Fas ligand (Fas-L, CD95L) were eight times more vulnerable to apoptosis than those not expressing Fas-L, which suggests that the T-cell apoptosis is induced by overactivation of the T-cell receptor, possibly in the absence of appropriate costimulation. Our results have implications for the design of immunotherapies for GBM. © 2006 Elsevier B.V. All rights reserved.




Walker, D. G., Chuah, T., Rist, M. J., & Pender, M. P. (2006). T-cell apoptosis in human glioblastoma multiforme: Implications for immunotherapy. Journal of Neuroimmunology, 175(1–2), 59–68. https://doi.org/10.1016/j.jneuroim.2006.03.006

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