Objective: By using a computerized vascular perfusion model, we investigated temporal effects of sub-acute pressure elevation on vasomotor behavior and expression of endothelin-1 (ET-1) and endothelial nitric oxide synthase (eNOS) in intact human conduit vessels. Methods: Paired umbilical veins were perfused during 1.5, 3 and 6 h under high/low intraluminal pressure (40/20 mmHg) and at identical shear stress level of 10 dyn/cm2. ET- 1 and eNOS gene and protein expression was quantified with real-time reverse- transcribed polymerase chain reaction and quantitative immunohistochemistry, respectively. Results: Pressure induced differential temporal regulation patterns of ET-1 and eNOS gene expression. During the high pressure condition, eNOS mRNA was upregulated after 3 h and leveled off after 6 h of perfusion, while ET-1 mRNA was elevated after 6 h perfusion. Immunohistochemistry verified synchronal changes at the protein level. Significant vasodilation was observed after 3 h in the high-pressure system. Conclusion: Thus, subacute pressure elevation exerts differential effects on the endothelial eNOS/ET-1 expression, which dynamically regulate the vasomotor tone. (C) 2000 Elsevier Science B.V. © Oxford University Press 2001.
Gan, L. M., Selin-Sjögren, L., Doroudi, R., & Jern, S. (2000). Temporal regulation of endothelial ET-1 and eNOS expression in intact human conduit vessels exposed to different intraluminal pressure levels at physiological shear stress. Cardiovascular Research, 48(1), 168–177. https://doi.org/10.1016/S0008-6363(00)00174-7