We describe the design of micropatterned surfaces for single cell studies, based on thermoresponsive polymer brushes. We show that brushes made of poly(N-isopropylacrylamide) grafted at high surface density display excellent protein and cell anti-adhesive properties. Such brushes are readily patterned at the micron scale via deep UV photolithography. A proper choice of the adhesive pattern shapes, combined with the temperature-dependent swelling properties of PNIPAM, allow us to use the polymer brush as a microactuator which induces cell detachment when the temperature is reduced below 32 ° C. © 2012 Mandal et al.
Mandal, K., Balland, M., & Bureau, L. (2012). Thermoresponsive micropatterned substrates for single cell studies. PLoS ONE, 7(5). https://doi.org/10.1371/journal.pone.0037548