Hepatitis C virus infects almost 170 million people per year but its assembly pathway, architecture and the structures of its envelope proteins are poorly understood. Using electron tomography of plastic-embedded sections of insect cells, we have visualized the morphogenesis of recombinant Hepatitis C virus-like particles. Our data provide a three-dimensional sketch of viral assembly at the endoplasmic reticulum showing different budding stages and contiguity of buds. This latter phenomenon could play an important role during the assembly of . wt-HCV and explain the size-heterogeneity of its particles. © 2012 Elsevier Inc..
Badia-Martinez, D., Peralta, B., Andrés, G., Guerra, M., Gil-Carton, D., & Abrescia, N. G. A. (2012). Three-dimensional visualization of forming Hepatitis C virus-like particles by electron-tomography. Virology, 430(2), 120–126. https://doi.org/10.1016/j.virol.2012.05.001