TLR expression profile of human gingival margin-derived stem progenitor cells

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Abstract

? Medicina Oral S. L. C.I.F.Background: Gingival margin-derived stem/progenitor cells (G-MSCs) show remarkable periodontal regenerative potential in vivo. During regeneration, G-MSCs may interact with their inflammatory environment via toll-like- receptors (TLRs). The present study aimed to depict the G-MSCs TLRs expression profile. Material and Methods: Cells were isolated from free gingival margins, STRO-1-immunomagnetically sorted and seeded to obtain single colony forming units (CFUs). G-MSCs were characterized for CD14, CD34, CD45, CD73, CD90, CD105, CD146 and STRO-1 expression, and for multilineage differentiation potential. Following G-MSCs? incubation in basic or inflammatory medium (IL-1?, IFN-?, IFN-?, TNF-?) a TLR expression profile was gene- rated.Results: G-MSCs showed all stem/progenitor cells? characteristics. In basic medium G-MSCs expressed TLRs 1, 2, 3, 4, 5, 6, 7, and 10. The inflammatory medium significantly up-regulated TLRs 1, 2, 4, 5, 7 and 10 and diminished TLR 6 (p?0.05, Wilcoxon-Signed-Ranks-Test).Conclusions: The current study describes for the first time the distinctive TLRs expression profile of G-MSCs under uninflamed and inflamed conditions.

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Fawzy-El-Sayed, K., Mekhemar, M., Adam-Klages, S., Kabelitz, D., & Dörfer, C. (2016). TLR expression profile of human gingival margin-derived stem progenitor cells. Medicina Oral, Patologia Oral y Cirugia Bucal, 21(1), e30–e38. https://doi.org/10.4317/medoral.20593

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