Tracing Asian Seabass Individuals to Single Fish Farms Using Microsatellites

9Citations
Citations of this article
23Readers
Mendeley users who have this article in their library.

Abstract

Traceability through physical labels is well established, but it is not highly reliable as physical labels can be easily changed or lost. Application of DNA markers to the traceability of food plays an increasingly important role for consumer protection and confidence building. In this study, we tested the efficiency of 16 polymorphic microsatellites and their combinations for tracing 368 fish to four populations where they originated. Using the maximum likelihood and Bayesian methods, three most efficient microsatellites were required to assign over 95% of fish to the correct populations. Selection of markers based on the assignment score estimated with the software WHICHLOCI was most effective in choosing markers for individual assignment, followed by the selection based on the allele number of individual markers. By combining rapid DNA extraction, and high-throughput genotyping of selected microsatellites, it is possible to conduct routine genetic traceability with high accuracy in Asian seabass. © 2012 Yue et al.

Cite

CITATION STYLE

APA

Yue, G. H., Xia, J. H., Liu, P., Liu, F., Sun, F., & Lin, G. (2012). Tracing Asian Seabass Individuals to Single Fish Farms Using Microsatellites. PLoS ONE, 7(12). https://doi.org/10.1371/journal.pone.0052721

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free