Trypanosoma cruzi survival following cold storage: Possible implications for tissue banking

7Citations
Citations of this article
20Readers
Mendeley users who have this article in their library.

Abstract

While Trypanosoma cruzi, the etiologic agent of Chagas disease, is typically vector-borne, infection can also occur through solid organ transplantation or transfusion of contaminated blood products. The ability of infected human cells, tissues, and cellular and tissue-based products (HCT/Ps) to transmit T. cruzi is dependent upon T. cruzi surviving the processing and storage conditions to which HCT/Ps are subjected. In the studies reported here, T. cruzi trypomastigotes remained infective 24 hours after being spiked into blood and stored at room temperature (N = 20); in 2 of 13 parasite-infected cultures stored 28 days at 4°C; and in samples stored 365 days at -80°C without cryoprotectant (N = 28), despite decreased viability compared to cryopreserved parasites. Detection of viable parasites after multiple freeze/thaws depended upon the duration of frozen storage. The ability of T. cruzi to survive long periods of storage at +4 and -80°C suggests that T. cruzi-infected tissues stored under these conditions are potentially infectious.

Cite

CITATION STYLE

APA

Martin, D. L., Goodhew, B., Czaicki, N., Foster, K., Rajbhandary, S., Hunter, S., & Brubaker, S. A. (2014). Trypanosoma cruzi survival following cold storage: Possible implications for tissue banking. PLoS ONE, 9(4). https://doi.org/10.1371/journal.pone.0095398

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free