Morphologically similar species such as Chinese black truffles and Tuber melanosporum were typed by restriction length polymorphism of internal transcribed spacers. This analysis together with sequence comparison revealed the presence of high genetic variability among fruit bodies collected in China. Selection of primer pairs allowed the internal transcribed spacer region of both Chinese truffles and T. melanosporum to be specifically amplified.
Paolocci, F., Rubini, A., Granetti, B., & Arcioni, S. (1997). Typing Tuber melanosporum and Chinese black truffle species by molecular markers. FEMS Microbiology Letters, 153(2), 255–260. https://doi.org/10.1016/S0378-1097(97)00226-7