Upregulation by retinoic acid of transforming growth factor-β-stimulated heat shock protein 27 induction in osteoblasts: Involvement of mitogen-activated protein kinases

38Citations
Citations of this article
6Readers
Mendeley users who have this article in their library.

Abstract

We investigated whether transforming growth factor-β (TGF-β) stimulates the induction of heat shock protein (HSP) 27 and HSP70 in osteoblast-like MC3T3-E1 cells and the mechanism underlying the induction. TGF-β increased the level of HSP27 but had no effect on the HSP70 level. TGF-β stimulated the accumulation of HSP27 dose-dependently, and induced an increase in the level of mRNA for HSP27. TGF-β induced the phosphorylation of p44/p42 mitogen-activated protein (MAP) kinase and p38 MAP kinase. The HSP27 accumulation induced by TGF-β was significantly suppressed by PD98059, an inhibitor of the upstream kinase of p44/p42 MAP kinase, or SB203580, an inhibitor of p38 MAP kinase. PD98059 and SB203580 suppressed the TGF-β-stimulated increase in the level of mRNA for HSP27. Retinoic acid, a vitamin A (retinol) metabolite, which alone had little effect on the HSP27 level, markedly enhanced the HSP27 accumulation stimulated by TGF-β. Retinoic acid enhanced the TGF-β-induced increase of mRNA for HSP27. The amplification of TGF-β-stimulated HSP27 accumulation by retinoic acid was reduced by PD98059 or SB203580. Retinoic acid failed to affect the TGF-β-induced phosphorylation of p44/p42 MAP kinase or p38 MAP kinase. These results strongly suggest that p44/p42 MAP kinase and p38 MAP kinase take part in the pathways of the TGF-β-stimulated HSP27 induction in osteoblasts, and that retinoic acid upregulates the TGF-β-stimulated HSP27 induction at a point downstream from p44/p42 MAP kinase and p38 MAP kinase. © 2002 Elsevier Science B.V. All rights reserved.

Cite

CITATION STYLE

APA

Hatakeyama, D., Kozawa, O., Niwa, M., Matsuno, H., Ito, H., Kato, K., … Uematsu, T. (2002). Upregulation by retinoic acid of transforming growth factor-β-stimulated heat shock protein 27 induction in osteoblasts: Involvement of mitogen-activated protein kinases. Biochimica et Biophysica Acta - Molecular Cell Research, 1589(1), 15–30. https://doi.org/10.1016/S0167-4889(01)00183-5

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free