The aim of this work was to study the anti- Candida activity of lidocaine and nitroglycerin alone and in combination. Ten Candida strains were included, corresponding to 1 collection type strain (ATCC 10231) and 9 clinical isolates: 4 C. albicans , 2 C. glabrata , 1 C. tropicalis , 1 C. krusei , and 1 C. parapsilosis . The CLSI reference M27-A3 micromethod was used to determine the anti- Candida activity of the drugs alone; minimal inhibitory and lethal concentrations were determined. The classic checkboard technique was used to determine the activity of combined drugs. Lidocaine fungicidal effect was dosedependent. Nitroglycerin exhibited a higher effect. The drugs combination resulted in a reduction of the inhibitory concentration, corresponding to an additive effect. In conclusion, both drugs exhibited an interesting anti- Candida activity. The combination of lidocaine with nitroglycerin was shown to have an additive effect against Candida spp., predicting the interest to include, in the future, these drugs in a new delivery system for the treatment of mucocutaneous candidosis.
Palmeira-de-Oliveira, A., Ramos, A. R., Gaspar, C., Palmeira-de-Oliveira, R., Gouveia, P., & Martinez-de-Oliveira, J. (2012). In Vitro Anti- Candida Activity of Lidocaine and Nitroglycerin: Alone and Combined . Infectious Diseases in Obstetrics and Gynecology, 2012, 1–4. https://doi.org/10.1155/2012/727248