In vitro and in vivo evaluation of polyherbal formulation against Russell's viper and cobra venom and screening of bioactive components by docking studies

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Abstract

<p> The present study emphasizes to reveal the antivenom activity of <italic>Aristolochia bracteolata</italic> Lam., <italic>Tylophora indica</italic> (Burm.f.) Merrill, and <italic>Leucas aspera S.</italic> which were evaluated against venoms of <italic>Daboia russelli russelli</italic> (Russell’s viper) and <italic>Naja naja</italic> (Indian cobra). The aqueous extracts of leaves and roots of the above-mentioned plants and their polyherbal (1 : 1 : 1) formulation at a dose of 200 mg/kg showed protection against envenomed mice with LD <sub>50</sub> doses of 0.44 mg/kg and 0.28 mg/kg against Russell’s viper and cobra venom, respectively. In <italic>in vitro</italic> antioxidant activities sample extracts showed free radical scavenging effects in dose dependent manner. Computational drug design and docking studies were carried out to predict the neutralizing principles of type I phospholipase A <sub>2</sub> (PLA <sub>2</sub> ) from Indian common krait venom. This confirmed that aristolochic acid and leucasin can neutralize type I PLA <sub>2</sub> enzyme. Results suggest that these plants could serve as a source of natural antioxidants and common antidote for snake bite. However, further studies are needed to identify the lead molecule responsible for antidote activity. </p>

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Sakthivel, G., Dey, A., Nongalleima, K., Chavali, M., Rimal Isaac, R. S., Singh, N. S., & Deb, L. (2013). In vitro and in vivo evaluation of polyherbal formulation against Russell’s viper and cobra venom and screening of bioactive components by docking studies. Evidence-Based Complementary and Alternative Medicine, 2013. https://doi.org/10.1155/2013/781216

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