For the first time, the use of fiber-optic color Doppler optical coherence tomography (CDOCT) to map in vivo the three-dimensional (3-D) vascular network of airway segments in human lungs is demonstrated. Visualizing the 3-D vascular network in the lungs may provide new opportunities for detecting and monitoring lung diseases such as asthma, chronic obstructive pulmonary disease, and lung cancer. Our CDOCT instrument employs a rotary fiberoptic probe that provides simultaneous two-dimensional (2-D) real-time structural optical coherence tomography (OCT) and CDOCT imaging at frame rates up to 12.5 frames per second. Controlled pullback of the probe allows 3-D vascular mapping in airway segments up to 50 mm in length in a single acquisition. We demonstrate the ability of CDOCT to map both small and large vessels. In one example, CDOCT imaging allows assignment of a feature in the structural OCT image as a large (∼1 mm diameter) blood vessel. In a second example, a smaller vessel (∼80 μm diameter) that is indistinguishable in the structural OCT image is fully visualized in 3-D using CDOCT. © 2013 The Authors.
Lee, A. M. D., Ohtani, K., MacAulay, C., McWilliams, A., Shaipanich, T., Yang, V. X. D., … Lane, P. (2013). In vivo lung microvasculature visualized in three dimensions using fiber-optic color Doppler optical coherence tomography . Journal of Biomedical Optics, 18(5), 050501. https://doi.org/10.1117/1.jbo.18.5.050501