Whole-Cell Recordings in Freely Moving Rats

172Citations
Citations of this article
484Readers
Mendeley users who have this article in their library.

Abstract

Intracellular recording, which allows direct measurement of the membrane potential and currents of individual neurons, requires a very mechanically stable preparation and has thus been limited to in vitro and head-immobilized in vivo experiments. This restriction constitutes a major obstacle for linking cellular and synaptic physiology with animal behavior. To overcome this limitation we have developed a method for performing whole-cell recordings in freely moving rats. We constructed a miniature head-mountable recording device, with mechanical stabilization achieved by anchoring the recording pipette rigidly in place after the whole-cell configuration is established. We obtain long-duration recordings (mean of ∼20 min, maximum 60 min) in freely moving animals that are remarkably insensitive to mechanical disturbances, then reconstruct the anatomy of the recorded cells. This head-anchored whole-cell recording technique will enable a wide range of new studies involving detailed measurement and manipulation of the physiological properties of identified cells during natural behaviors. © 2006 Elsevier Inc. All rights reserved.

Author supplied keywords

Cite

CITATION STYLE

APA

Lee, A. K., Manns, I. D., Sakmann, B., & Brecht, M. (2006). Whole-Cell Recordings in Freely Moving Rats. Neuron, 51(4), 399–407. https://doi.org/10.1016/j.neuron.2006.07.004

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free