X-ray scattering of vesicles of N-acyl sphingomyelins. Determination of bilayer thickness

56Citations
Citations of this article
20Readers
Mendeley users who have this article in their library.

Abstract

A series of N-acyl sphingomyelins (C16:0, C18:0, C20:0, C22:0, and C24:0) have been synthesized and single bilayer vesicles formed by sonication and ultracentrifugation. X-ray scattering data have been collected from the sphingomyelin vesicles at 50 degrees C in the melted-chain state. The x-ray scattering data have been transformed to the corresponding Patterson functions and Fourier electron density profiles; analysis of these functions has provided the intrabilayer phosphate-phosphate separation dp-p, a measure of the lipid bilayer thickness. The bilayer thickness increases linearly with increasing chain length (increment 1.3–1.4 A) and the intercept, 14.3–15.0 A, suggests a contribution of 7.0–7.5 A for each phosphorylcholine group to the bilayer thickness. The electron-density profiles have features suggestive of chain interdigitation when the length of the N-acyl chain (C20:0, C22:0, and C24:0) exceeds significantly the length of the invariant sphingosine chain. © 1986, The Biophysical Society. All rights reserved.

Cite

CITATION STYLE

APA

Maulik, P. R., Atkinson, D., & Shipley, G. G. (1986). X-ray scattering of vesicles of N-acyl sphingomyelins. Determination of bilayer thickness. Biophysical Journal, 50(6), 1071–1077. https://doi.org/10.1016/S0006-3495(86)83551-2

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free