We report for the first time the cloning and characterisation of a protozoal enzyme involved in plant cell wall polysaccharide degradation. A cDNA library was constructed from the ruminal protozoan Polyplastron multivesiculatum and a stable clone expressing xylanase activity was isolated. The encoded enzyme belongs to the glycoside hydrolase family 11, and phylogenetic analysis indicates a closer relationship with catalytic domains from Gram-positive bacteria than the other fibrolytic eukaryotes from the rumen, the anaerobic fungi. Copyright (C) 1999.
Devillard, E., Newbold, C. J., Scott, K. P., Forano, E., Wallace, R. J., Jouany, J. P., & Flint, H. J. (1999). A xylanase produced by the rumen anaerobic protozoan Polyplastron multivesiculatum shows close sequence similarity to family 11 xylanases from Gram-positive bacteria. FEMS Microbiology Letters, 181(1), 145–152. https://doi.org/10.1016/S0378-1097(99)00525-X