Proper spindle positioning is essential for spatial control of cell division. Here, we show that zyg-8 plays a key role in spindle positioning during asymmetric division of one-cell stage C. elegans embryos by promoting microtubule assembly during anaphase. ZYG-8 harbors a kinase domain and a domain related to Doublecortin, a microtubule-associated protein (MAP) affected in patients with neuronal migration disorders. Sequencing of zyg-8 mutant alleles demonstrates that both domains are essential for function. ZYG-8 binds to microtubules in vitro, colocalizes with microtubules in vivo, and promotes stabilization of microtubules to drug or cold depolymerization in COS-7 cells. Our findings demonstrate that ZYG-8 is a MAP crucial for proper spindle positioning in C. elegans, and indicate that the function of the Doublecortin domain in modulating microtubule dynamics is conserved across metazoan evolution. © 2001 Cell Press.
Gönczy, P., Bellanger, J. M., Kirkham, M., Pozniakowski, A., Baumer, K., Phillips, J. B., & Hyman, A. A. (2001). Zyg-8, a Gene Required for Spindle Positioning in C. elegans, Encodes a Doublecortin-Related Kinase that Promotes Microtubule Assembly. Developmental Cell, 1(3), 363–375. https://doi.org/10.1016/S1534-5807(01)00046-6