β-oxidation of medium chain (C8C14) fatty acids studied in isolated liver cells

  • Christensen E
  • Hagve T
  • Grønn M
 et al. 
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Abstract

The β-oxidation and esterification of medium-chain fatty acids were studied in hepatocytes from fasted, fed and fructose-refed rats. The β-oxidation of lauric acid (12:0) was less inhibited by fructose refeeding and by (+)-decanoyl-carnitine than the oxidation of oleic acid was, suggesting a peroxisomal β-oxidation of lauric acid. Little lauric acid was esterified in triacylglycerol fraction, except at high substrate concentrations or in the fructose-refed state. With [1-14C]myristic acid (14:0), [1-14C]lauric acid (12:0), [1-14C]octanoic acid (8:0) and [2-14C]adrenic acid (22:4(n - 6)) as substrate for hepatocytes from carbohydrate-refed rats, a large fraction of the14C-labelled esterified fatty acids consisted of newly synthesized palmitic acid (16:0), stearic acid (18:0) and oleic acid (18:1) while intact [1-14C]oleic acid substrate was esterified directly. With [9,10-3H]myristic acid as the substrate, small amounts of shortened3H-labelled β-oxidation intermediates were found. With [U-14C]palmitic acid, no shortened fatty acids were detected. It was concluded that when the mitochondrial fatty acid oxidation is down-regulated such as in the carbohydrate-refed state, medium-chain fatty acids can partly be retailored to long-chain fatty acids by peroxisomal β-oxidation followed by synthesis of C16and C16fatty acids which can then stored as triacylglycerol. © 1989.

Author-supplied keywords

  • (Peroxisome)
  • (Rat hepatocyte)
  • Fatty acid oxidation
  • Lauric acid
  • Mitochondrion
  • Myristic acid
  • Octanoic acid

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Authors

  • Erik Christensen

  • Tor Arne Hagve

  • Morten Grønn

  • Bjørn O. Christophersen

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