A phage genome has been isolated which contains both attB and attP, the bacterial and phage sites which are recombined in the process of formation of stable lysogens. The derivative was created by an illegitimate recombination during mixed vegetative growth of two phage strains which contained attB and attP separately. The attachment sites on the double site derivative can undergo site-specific recombination to to yield a recombinant deleted for about 15% of the parental DNA. This recombination is dependent on the product of the int gene and demonstrates a great preference for recombination between two sites located on the same molecule. The int gene product used for recombination of λattB-attP may be provided either from the int gene residing either on λattB-attP or on another phage genome. In both cases, ochre suppression of amber mutations of the int gene results in extremely depressed levels of integrative recombination. © 1974.
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