15N relaxation and quantification of 15N-labelled metabolites in cell extracts

  • Houyou N
  • Pau-Roblot C
  • Roscher A
  • 2


    Mendeley users who have this article in their library.
  • 0


    Citations of this article.


15N is useful for studying nitrogen metabolism by isotope labelling. However, the15N NMR analysis is hampered by negative nOe factors and sometimes long T1values. Moreover, these can have a marked dependency on their physico-chemical environment (pH, ionic charge, viscosity, etc.) making quantitative analysis of cell extracts from labelling experiments difficult. We have, therefore, analysed some of these dependencies in order to find conditions in which quantitative analysis of some major metabolites (amino acids, ammonium, nitrate, urea) can be performed reliably and with sufficient signal. © 2005 Académie des sciences. Published by Elsevier SAS. All rights reserved.

Author-supplied keywords

  • Cell extracts
  • NMR
  • Nitrogen metabolism
  • Nitrogen-15
  • Nuclear Overhauser effect (nOe)
  • Relaxation T1
  • Stable isotope labelling

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Nassima Houyou

  • Corinne Pau-Roblot

  • Albrecht Roscher

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free