Citations of this article
Mendeley users who have this article in their library.
Get full text


This chapter discusses the analysis of refractile (R) bodies that are bacterial inclusion bodies consisting of a convoluted proteinaceous ribbon, found naturally in Caedibacter and in some strains of the genus Pseudomonas. For most investigations on R bodies it is necessary to purify them to some extent or to homogeneity. In all investigations, it is recommended to start with cultures having a high ratio of R body-containing cells. Negative staining is used preferentially for investigating isolated R bodies and also for detecting R bodies inside cells. Metal shadowing is used for detecting R bodies inside whole cells and for examining isolated R bodies in the extended form, allowing the differentiation of two surfaces, one with a more granular texture in the inner side and the other, at the outer surface, relatively smooth. Ultra-thin sections of whole cells show that the R bodies are rolled-up double membrane systems with two electron-dense zones. The average thickness is 11–16 nm. Most of the R bodies sectioned contain a fibrous core material or spherical bodies, which could be associated with their assembly. © 1988, Elsevier Ltd. All rights reserved.




Lalucat, J. (1988). 4 Analysis of Refractile (R) Bodies. Methods in Microbiology, 20(C), 79–90. https://doi.org/10.1016/S0580-9517(08)70048-5

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free