Adsorption, retention and biologic activity of proteins adsorbed on gas discharge treated surfaces

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Abstract

We have observed that proteins adsorb very tenaciously to polymer surfaces that have been treated with certain gases in a gas discharge. These gases include tetrachoroethylene (TFE) and tetrachloroethylene (TCE). We have studied adsorption of plasma proteins, antibodies, antigens and enzymes. In this article we present results on the adsorption, retention and biologic activity of (a) albumin as a passivating surface, (b) malaria and schistosomiasis antibodies in an immunoassay and (c) horseradish peroxidase (HRP) as a model enzyme biosensor. In some cases, the protein adsorbed to the treated surface retains greater activity and/or greater stability when compared to the untreated control surface. We also discuss the possible mechanism(s) of these interesting and unusual effects. © 1992.

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Hoffman, A. S., Kiaei, D., Safranj, A., Chen, J. P., Johnstona, A., Horbett, T. A., … Markovic, V. (1992). Adsorption, retention and biologic activity of proteins adsorbed on gas discharge treated surfaces. Clinical Materials, 11(1–4), 93–98. https://doi.org/10.1016/0267-6605(92)90033-P

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