Allele frequencies of 15 STR loci in a Spanish population

Abstract

Genetic analysis of 15 STR loci, including the 13 CODIS core STR loci (D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820, TH01, TPOX, CSF1PO, and D16S539) and the 2 pentameric STRs (Penta D and Penta E) present in the Powerplex 16™ system (Promega, Madison, WI, USA) were carried out. Blood and/or buccal swab samples were obtained from 341 unrelated Spanish individuals and DNA was extracted using phenol/chloroform and ethanol precipitation. The 15 STR loci plus de Amelogenin locus were electrophoresed using the ABI Prism 310 Automatic DNA Sequencer and alleles were typed using GeneScan and Genotyper software (Applied Biosystems). Allele frequencies for each locus were calculated and the exact test and the homozygosity test were performed to evaluate Hardy–Weinberg equilibrium (HWE). The Expected Heterozygosity (He), the Observed Heterozygosity (Ho), the Polymorphic Information Content (PIC), the Power of Discrimination (PD) and the Power of Exclusion (PE) were also calculated. The results indicate that these 15 loci are useful genetic markers for forensic personal identification and paternity testing in the Spanish population. © 2003 Elsevier B.V.