The activity of the enzyme, acetolactate synthase, extracted from cultured, wild-type cells of Datura innoxia P. Mill. was inhibited by valine and leucine (5-20 mM), added either separately or together, and by chlorsulfuron (10-8 and 10-5 M). Isoleucine (5-20 mM) was a weaker inhibitor than valine and leucine. Acetolactate synthase isolated from four chlorsulfuron-resistant, D. innoxia cell variants was also tested for feedback sensitivity to the three amino acids. Results showed that the acetolactate synthase from chlorsulfuron-resistant variants had less feedback sensitivity to valine, leucine and isoleucine added to assay mixtures either separately or in combination, compared to the enzyme from wild-type cells. © 1990.
Rathinasabapathi, B., Williams, D., & King, J. (1990). Altered feedback sensitivity to valine, leucine and isoleucine of acetolactate synthase from herbicide-resistant variants of Datura innoxia. Plant Science, 67(1), 1–6. https://doi.org/10.1016/0168-9452(90)90044-O