Amperometric immunosensing using microperoxidase MP-11 antibody conjugates

  • Padeste C
  • Grubelnik A
  • Tiefenauer L
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Abstract

Electrochemical immunosensing requires labeling because most antigen and antibody species are electrochemically inert. The use of microperoxidase as the label is most promising due to its electrocatalytic activity, which is utilized for signal amplification. A method for coupling the microperoxidase MP-11 to antibody molecules has been developed. The electrocatalytic effect of the conjugate when immobilized on gold electrodes has been demonstrated. Using the streptavidin-biotin system the antigen estradiol was immobilized on gold. Recognition of labeled antibody could be detected electrochemically. Copyright (C) 1998 Elsevier Science B.V.

Author-supplied keywords

  • Electrocatalytic reduction
  • Immunosensor
  • Microperoxidase

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Authors

  • Celestino Padeste

  • Andreas Grubelnik

  • Louis Tiefenauer

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