Analysis of the interaction between GGA1 GAT domain and rabaptin-5

  • Zhu G
  • Zhai P
  • Wakeham N
 et al. 
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GGAs are a family of adaptor proteins involved in vesicular transport. As an effector of the small GTPase Arf, GGA interacts using its GAT domain with the GTP-bound form of Arf. The GAT domain is also found to interact with ubiquitin and rabaptin-5. Rabaptin-5 is, in turn, an effector of another small GTPase, Rab5, which regulates early endosome fusion. The interaction between GGAs and rabaptin-5 is likely to take place in a pathway between the trans-Golgi network and early endosomes. This chapter describes in vitro biochemical characterization of the interaction between the GGA1 GAT domain and rabaptin-5. Combining with the complex crystal structure, we reveal that the binding mode is helix bundle-to-helix bundle in nature. Copyright 2005, Elsevier Inc. All rights reserved.

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  • Guangyu Zhu

  • Peng Zhai

  • Nancy Wakeham

  • Xiangyuan He

  • Xuejun C. Zhang

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