Anti-idiotype technique: An alternative approach for immunodiagnosis of bluetongue

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Abstract

In development of a bluetongue alternative immunodiagnostic rest, the polyclonal anti-idiotypic antibodies were generated by the sequential immunization of rabbits with three monoclonal antibodies to VP7 of bluetongue virus. The anti-idiotypic antibodies recognize the idiotypes that are located within or near the antigen-combining sites and are associated with both heavy and light chains of the antibodies to VP7 of bluetongue virus. The anti- idiotypic antibodies mimic the VP7 antigen by recognizing the anti-VP7 antibodies from cattle and sheep that were infected with various serotypes of bluetongue viruses. The results indicate that the rabbit anti-idiotypic antibodies may be used as surrogate antigen in serological assays to detect the antibodies from different species of animals infected with various serotypes of bluetongue viruses.

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Zhou, E. M. (1999). Anti-idiotype technique: An alternative approach for immunodiagnosis of bluetongue. Veterinary Immunology and Immunopathology, 72(1–2), 237–242. https://doi.org/10.1016/S0165-2427(99)00137-3

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