An apparatus which facilitates immunoelectrofocusing is described. Polyacrylamide is used as the support medium for both the thin-layer isoelectric focusing and immunodiffusion stages. The pH gradients formed during electrofocusing are reproducible and sufficiently stable. Immunoelectrofocused and isoelectric focused patterns indicate that at least one inactive form and four active forms of squid tyrosinase exist. © 1978.
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