Growth at the restrictive temperature (42°C) of Aspergillus nidulans B120, carrying the conditional-lethal mutation sodVIC1, was partially improved by the addition of 1.0 M sorbitol to the medium. The mutant grown at 42°C, with osmotic stabilizer, showed abnormal hyphal morphology, a decrease in β-1,3-glucan synthase activity as well as cell wall sugar content, but an increase in chitin synthase activity and N-acetyl-glucosamine content. The mutation also affected the secretion of extracellular protease. The temperature-dependent osmo-sensitive phenotype of a Saccharomyces cerevisiae α-COP mutation can be rescued by the A. nidulans sodVIC+gene. These results indicate that the sodVIC1 mutation affects proper processing of secretory proteins destined for the surface of cells or beyond. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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