Asymmetric lipid bilayers are formed by adjoining the hydrocarbon chains of two different lipid monolayers at the air-water interface through an aperture in a teflon partition separating two aqueous phases. It is shown that the addition of Ca2+or polysine to the compartment limited by a monolayer of the neutral lipids glycerol dioleate or phosphatidylcholine results in no modification of the resistance and stability of the membrane, whereas a drastic decrease in both parameters is elicited by the presence of these ions on the opposite compartment containing a monolayer of the negatively charged cardiolipin or phosphatidylserine. The surface-charge dependence of this phenomenon indicates the persistence of the asymmetric lipid distribution in the bilayer after its formation from two different monolayers. © 1973.
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