Nucleotide measurements have been used as biomass indicators in microbial ecology. We report the use of this metric in materially closed, energetically open microbial ecosystems. Our results indicate that: (i) both ATP concentrations and pO2show an increase from time of closure; (ii) ATP concentrations (0.2 1.2 ng/ml) are approximately 2-3 times higher than open ocean water; (iii) there is an apparent oscillation of ATP concentrations on the order of 50 days; and (iv) within experimental limitations the ratios of ATP concentrations to observed total cell counts is constant. © 1983.
Takano, C. T., Folsome, C. E., & Karl, D. M. (1983). ATP as a biomass indicator for closed ecosystems. BioSystems, 16(1), 75–78. https://doi.org/10.1016/0303-2647(83)90027-8