Automated large-volume sample stacking procedure to detect labeled peptides at picomolar concentration using capillary electrophoresis and laser-induced fluorescence detection

  • Siri N
  • Riolet P
  • Bayle C
 et al. 
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Abstract

We have developed an automated large-volume sample stacking (LVSS) procedure to detect fluorescein isothiocyanate-labeled peptides in the picomolar range. The injection duration is 10 min at 50 mbar to fill 62% of the capillary volume to the detection cell. The calculated limit of detection (S/N=3), filling 1% of the capillary volume, is 74 pM for bradykinin and 45 pM for L-enkephalin with samples diluted in water and analyzed in a 50 mM borate buffer, pH 9.2. With the automated LVSS system, the limits of detection are 7 pM for bradykinin, 3 pM for L-enkephalin and 2 pM for substance P. LVSS is shown to be quantitative from 500 to 10 pM. © 2003 Elsevier B.V. All rights reserved.

Author-supplied keywords

  • Fluorescein isothiocyanate
  • Large-volume sample stacking
  • Peptides

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Authors

  • Nathalie Siri

  • Pierre Riolet

  • Christophe Bayle

  • François Couderc

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