Azide has no effect on photophosphorylation at normal light intensities, inhibits acid-base ATP synthesis in the dark weakly, and is a moderately strong inhibitor of the ATP-Piexchange reaction occurring in the dark after activation of spinach thylakoids by light. However, azide is a potent inhibitor of the methanol-activated ATPase of thylakoids (which is not associated with proton pumping). When ATPase has been activated by light plus dithiothreitol it pumps protons during ATP hydrolysis, so the thylakoid high-energy state is maintained in darkness. Azide has no effect on this reaction. When the light + dithiothreitol-activated ATPase is maximally stimulated by the uncoupler, NH4Cl, azide becomes a strong inhibitor. We suggest that inhibition of ATPase by azide occurs only when the net thylakoid protonmotive force differential is less than maximal. © 1988.
Wei, J. mian, Howlett, B., & Jagendorf, A. T. (1988). Azide inhibition of chloroplast ATPase is prevented by a high protonmotive force. BBA - Bioenergetics, 934(1), 72–79. https://doi.org/10.1016/0005-2728(88)90121-1