Azide inhibition of chloroplast ATPase is prevented by a high protonmotive force

  • Wei J
  • Howlett B
  • Jagendorf A
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Abstract

Azide has no effect on photophosphorylation at normal light intensities, inhibits acid-base ATP synthesis in the dark weakly, and is a moderately strong inhibitor of the ATP-Piexchange reaction occurring in the dark after activation of spinach thylakoids by light. However, azide is a potent inhibitor of the methanol-activated ATPase of thylakoids (which is not associated with proton pumping). When ATPase has been activated by light plus dithiothreitol it pumps protons during ATP hydrolysis, so the thylakoid high-energy state is maintained in darkness. Azide has no effect on this reaction. When the light + dithiothreitol-activated ATPase is maximally stimulated by the uncoupler, NH4Cl, azide becomes a strong inhibitor. We suggest that inhibition of ATPase by azide occurs only when the net thylakoid protonmotive force differential is less than maximal. © 1988.

Author-supplied keywords

  • ATPase
  • Azide inhibition
  • CF1
  • Chloroplast
  • Protonmotive force
  • Thylakoid

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Authors

  • Jia mian Wei

  • Bruce Howlett

  • AndréT T. Jagendorf

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